Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 60,222 bioRxiv papers from 267,720 authors.

Molecular anatomy and plasticity of the long noncoding RNA HOTAIR

By Rachel Spokoini-Stern, Dimitar Stamov, Hadass Jessel, Lior Aharoni, Heiko Haschke, Jonathan Giron, Ron Unger, Eran Segal, Almogit Abu-Horowitz, Ido Bachelet

Posted 15 Oct 2018
bioRxiv DOI: 10.1101/444331

Long noncoding RNA molecules (lncRNAs) are estimated to account for the majority of eukaryotic genomic transcripts, and have been associated with multiple diseases in humans. However, our understanding of their structure-function relationships is scarce, with structural evidence coming mostly from indirect biochemical approaches or computational predictions. Here we describe the hypothetical molecular anatomy of the lncRNA HOTAIR (HOx Transcript AntIsense RNA) inferred from direct, high-resolution visualization by atomic force microscopy (AFM) in nucleus-like conditions at 37 degrees. Our observations reveal that HOTAIR has a distinct anatomy with a high degree of plasticity. Fast AFM scanning enabled the quantification of this plasticity, and provided visual evidence of physical interactions with genomic DNA segments. Our report provides the first biologically-plausible hypothetical description of the anatomy and intrinsic properties of HOTAIR, and presents a framework for studying the structural biology of lncRNAs.

Download data

  • Downloaded 541 times
  • Download rankings, all-time:
    • Site-wide: 16,656 out of 60,222
    • In biophysics: 555 out of 2,550
  • Year to date:
    • Site-wide: 6,608 out of 60,222
  • Since beginning of last month:
    • Site-wide: 20,143 out of 60,222

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)