Electron Cryo-Tomography Provides Insight into Procentriole Architecture and Assembly Mechanism

Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from Chlamydomonas reinhardtii. We identified a set of non-tubulin components attached to the triplet microtubule (MT), many are at the junctions of tubules likely to reinforce the triplet. We describe structure of the A-C linker that bridges neighboring triplets. We find that POC1 is an integral component of the A-C linker. Its conserved WD40 β-propeller domain provides sites for attachment to other A-C linker components. The twist of A-C linker results in an iris diaphragm-like motion of the triplets in the longitudinal direction of procentriole. Finally, we identified two assembly intermediates at the growing ends of procentriole allowing us to propose a model for the procentriole assembly. Our results provide a comprehensive structural framework for understanding the molecular mechanisms underpinning procentriole biogenesis and assembly.

Download data

• Downloaded 569 times
• Download rankings, all-time:
  • Site-wide: 37,287 out of 118,665
  • In cell biology: 1,559 out of 5,436
• Year to date:
  • Site-wide: 103,510 out of 118,665
• Since beginning of last month:
  • Site-wide: 103,855 out of 118,665

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide