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A cellular anatomy of the normal adult human prostate and prostatic urethra

By Gervaise H. Henry, Alicia Malewska, Diya B. Joseph, Venkat Malladi, Jeon Lee, Jose Torrealba, Ryan J. Mauck, Jeffrey C. Gahan, Ganesh V Raj, Claus G. Roehrborn, Gary C. Hon, Malcolm P. MacConmara, Jeffrey C. Reese, Ryan C. Hutchinson, Chad M Vezina, Douglas W. Strand

Posted 10 Oct 2018
bioRxiv DOI: 10.1101/439935 (published DOI: 10.1016/j.celrep.2018.11.086)

A cellular anatomy of normal human organs is essential for solving the cellular origins of disease. We report the first comprehensive cellular atlas of the young adult human prostate and prostatic urethra using an iterative process of single cell RNA sequencing and flow cytometry on ~98,000 cells taken from different anatomical regions. Two previously unrecognized epithelial cell types were identified by KRT13 and SCGB1A1 expression and found to be highly similar to hillock and club cells of the proximal lung. It was demonstrated by immunohistochemistry that prostate club and hillock cells are similarly concentrated in the proximal prostate. We also optimized a new flow cytometry antibody panel to improve cell type-specific purification based on newly established cellular markers. The molecular classification, anatomical distribution, and purification methods for each cell type in the human prostate create a powerful new resource for experimental design in human prostate disease.

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