Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,118 bioRxiv papers from 295,271 authors.
Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response.
By
Jieqiong Lou,
Lorenzo Scipioni,
Belinda K Wright,
Tara K Bartolec,
Jessie Zhang,
V Pragathi Masamsetti,
Katharina Gaus,
Enrico Gratton,
Anthony J Cesare,
Elizabeth Hinde
Posted 17 Sep 2018
bioRxiv DOI: 10.1101/419523
(published DOI: 10.1073/pnas.1814965116)
To investigate how chromatin architecture is spatiotemporally organised at a double strand break (DSB) repair locus, we established a biophysical method to quantify chromatin compaction at the nucleosome level during the DNA damage response (DDR). The method is based on phasor image correlation spectroscopy (ICS) of histone FLIM-FRET microscopy data acquired in live cells co-expressing H2B-eGFP and H2B-mCherry. This multiplexed approach generates spatiotemporal maps of nuclear-wide chromatin compaction that when coupled with laser micro-irradiation induced DSBs, quantify the size, stability, and spacing between compact chromatin foci throughout the DDR. Using this technology, we identify that ATM and RNF8 regulate rapid chromatin decompaction at DSBs and formation of a compact chromatin ring surrounding the repair locus. This chromatin architecture serves to demarcate the repair locus from the surrounding nuclear environment and modulate 53BP1 mobility.
Download data
- Downloaded 478 times
- Download rankings, all-time:
- Site-wide: 22,180 out of 67,095
- In biophysics: 803 out of 2,842
- Year to date:
- Site-wide: 38,293 out of 67,095
- Since beginning of last month:
- Site-wide: 47,149 out of 67,095
Altmetric data
Downloads over time
Distribution of downloads per paper, site-wide
- Home
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- About
News
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!