Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 54,982 bioRxiv papers from 253,713 authors.

RELION-3: new tools for automated high-resolution cryo-EM structure determination

By Jasenko Zivanov, Takanori Nakane, Bjorn Forsberg, Dari Kimanius, Wim J.H. Hagen, Erik Lindahl, Sjors Scheres

Posted 19 Sep 2018
bioRxiv DOI: 10.1101/421123 (published DOI: 10.7554/eLife.42166)

Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations. Reference-free autopicking with Laplacian-of-Gaussian filtering and execution of jobs from python allows non-interactive processing during acquisition, including 2D-classification, de novo model generation and 3D-classification. Per-particle refinement of CTF parameters and correction of estimated beam tilt provides higher-resolution reconstructions when particles are at different heights in the ice, and/or coma-free alignment has not been optimal. Ewald sphere curvature correction improves resolution for large particles. We illustrate these developments with publicly available data sets: together with a Bayesian approach to beam-induced motion correction it leads to resolution improvements of 0.2-0.7 Å compared to previous RELION versions.

Download data

  • Downloaded 5,292 times
  • Download rankings, all-time:
    • Site-wide: 307 out of 54,982
    • In biophysics: 5 out of 2,327
  • Year to date:
    • Site-wide: 109 out of 54,982
  • Since beginning of last month:
    • Site-wide: 175 out of 54,982

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)