Rxivist logo

RELION-3: new tools for automated high-resolution cryo-EM structure determination

By Jasenko Zivanov, Takanori Nakane, BO Forsberg, Dari Kimanius, Wim J. H. Hagen, Erik Lindahl, Sjors Scheres

Posted 19 Sep 2018
bioRxiv DOI: 10.1101/421123 (published DOI: 10.7554/eLife.42166)

Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations. Reference-free autopicking with Laplacian-of-Gaussian filtering and execution of jobs from python allows non-interactive processing during acquisition, including 2D-classification, de novo model generation and 3D-classification. Per-particle refinement of CTF parameters and correction of estimated beam tilt provides higher-resolution reconstructions when particles are at different heights in the ice, and/or coma-free alignment has not been optimal. Ewald sphere curvature correction improves resolution for large particles. We illustrate these developments with publicly available data sets: together with a Bayesian approach to beam-induced motion correction it leads to resolution improvements of 0.2-0.7 Å compared to previous RELION versions.

Download data

  • Downloaded 9,982 times
  • Download rankings, all-time:
    • Site-wide: 1,049
    • In biophysics: 7
  • Year to date:
    • Site-wide: 5,941
  • Since beginning of last month:
    • Site-wide: 20,198

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News