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Analysis and comparison of genome editing using CRISPResso2
Matthew C. Canver,
Jason M. Gehrke,
Jonathan Y Hsu,
David R Liu,
J. Keith Joung,
Daniel E. Bauer,
Posted 15 Aug 2018
bioRxiv DOI: 10.1101/392217 (published DOI: 10.1038/s41587-019-0032-3)
Posted 15 Aug 2018
Genome editing technologies are rapidly evolving, and analysis of deep sequencing data from target or off-target regions is necessary for measuring editing efficiency and evaluating safety. However, no software exists to analyze base editors, perform allele-specific quantification or that incorporates biologically-informed and scalable alignment approaches. Here, we present CRISPResso2 to fill this gap and illustrate its functionality by experimentally measuring and analyzing the editing properties of six genome editing agents.
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