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High-throughput single-cell transcriptome profiling of plant cell types

By Christine N Shulse, Benjamin J. Cole, Gina M Turco, Yiwen Zhu, Siobhan M. Brady, Diane E. Dickel

Posted 29 Aug 2018
bioRxiv DOI: 10.1101/402966 (published DOI: 10.1016/j.celrep.2019.04.054)

Single-cell transcriptome analysis of heterogeneous tissues can provide high-resolution windows into the genomic basis and spatiotemporal dynamics of developmental processes. Here we demonstrate the feasibility of high-throughput single-cell RNA sequencing of plant tissue using the Drop-seq approach. Profiling of >4,000 individual cells from the Arabidopsis root provides transcriptomes and marker genes for a diversity of cell types and illuminates the gene expression changes that occur across endodermis development.

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