Three-Dimensional Histology of Whole Zebrafish by Sub-Micron Synchrotron X-ray Micro-Tomography
Daniel J. Vanselow,
Maksim A. Yakovlev,
Spencer R. Katz,
Alex Y Lin,
Darin P Clark,
Jean E Copper,
Victor A Canfield,
Khai C Ang,
Francesco De Carlo,
Damian B. van Rossum,
Patrick La Rivière,
Keith C. Cheng
Posted 25 Aug 2018
bioRxiv DOI: 10.1101/392381 (published DOI: 10.7554/eLife.44898)
Posted 25 Aug 2018
Histological studies providing cellular insights into tissue architecture have been central to biological discovery and remain clinically invaluable today. Extending histology to three dimensions would be transformational for research and diagnostics. However, three-dimensional histology is impractical using current techniques. We have customized sample preparation, synchrotron X-ray tomographic parameters, and three-dimensional image analysis to allow for complete histological phenotyping using whole larval and juvenile zebrafish. The resulting digital zebrafish can be virtually sectioned and visualized in any plane. Whole-animal reconstructions at subcellular resolution also enable computational characterization of the zebrafish nervous system by region-specific detection of cell nuclei and quantitative assessment of individual phenotypic variation. Three-dimensional histological phenotyping has potential use in genetic and chemical screens, and in clinical and toxicological tissue diagnostics.
- Downloaded 2,320 times
- Download rankings, all-time:
- Site-wide: 2,979 out of 92,645
- In pathology: 18 out of 532
- Year to date:
- Site-wide: 16,348 out of 92,645
- Since beginning of last month:
- Site-wide: 24,358 out of 92,645
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!