Mapping the Conformational Landscape of a Dynamic Enzyme by XFEL and Multitemperature Crystallography
Daniel A. Keedy,
Lillian R. Kenner,
Rahel A. Woldeyes,
Jesse B Hopkins,
Michael C Thompson,
Aaron S. Brewster,
Andrew H Van Benschoten,
Elizabeth L. Baxter,
Scott E McPhillps,
James M. Holton,
William I. Weis,
Axel T Brunger,
S. Michael Soltis,
Nicholas K Sauter,
Aina E. Cohen,
Henry van den Bedem,
Robert E. Thorne,
James S Fraser
Posted 19 Mar 2015
bioRxiv DOI: 10.1101/016733 (published DOI: 10.7554/eLife.07574)
Posted 19 Mar 2015
Determining the interconverting conformations of dynamic proteins in atomic detail is a major challenge for structural biology. Conformational heterogeneity in the active site of the dynamic enzyme cyclophilin A (CypA) has been previously linked to its catalytic function. Here we compare the conformational ensembles of CypA by fixed-target X-ray free electron laser (XFEL) crystallography and multitemperature synchrotron crystallography. The “diffraction-before-destruction” nature of XFEL experiments provides a radiation-damage-free view of the functionally important alternative conformations of CypA. We monitored the temperature dependences of these alternative conformations with eight synchrotron datasets spanning 100-310 K. Multiconformer models show that many alternative conformations in CypA are populated above, but not below, the glass transition temperature (~200 K) and reveal abrupt changes in protein flexibility that provide all-atom insight into conformational coupling. Together, our XFEL data and multitemperature analyses motivate a new generation of time-resolved experiments to structurally characterize the dynamic underpinnings of protein function.
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