Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,897 bioRxiv papers from 309,427 authors.

Chromosome-wide simulations uncover folding pathway and 3D organization of interphase chromosomes

By Davide Michieletto, Davide Marenduzzo, Ajazul H. Wani

Posted 12 Apr 2016
bioRxiv DOI: 10.1101/048116

Three-dimensional interphase organization of metazoan genomes has been linked to cellular identity. However, the principles governing 3D interphase genome architecture and its faithful transmission through disruptive events of cell-cycle, like mitosis, are not fully understood. By using Brownian dynamics simulations of Drosophila chromosome 3R up to time-scales of minutes, we show that chromatin binding profile of Polycomb-repressive-complex-1 robustly predicts a sub-set of topologically associated domains (TADs), and inclusion of other factors recapitulates the profile of all TADs, as observed experimentally. Our simulations show that chromosome 3R attains interphase organization from mitotic state by a two-step process in which formation of local TADs is followed by long-range interactions. Our model also explains statistical features and tracks the assembly kinetics of polycomb subnuclear clusters. In conclusion, our approach can be used to predict structural and kinetic features of 3D chromosome folding and its associated proteins in biological relevant genomic and time scales.

Download data

  • Downloaded 438 times
  • Download rankings, all-time:
    • Site-wide: 26,718 out of 70,861
    • In biophysics: 1,021 out of 3,020
  • Year to date:
    • Site-wide: 68,694 out of 70,861
  • Since beginning of last month:
    • Site-wide: 13,474 out of 70,861

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News