Rxivist logo

A pipeline approach to single-particle processing in RELION

By Rafael Fernandez-Leiro, Sjors Scheres

Posted 29 Sep 2016
bioRxiv DOI: 10.1101/078352 (published DOI: 10.1107/S2059798316019276)

We describe the formal concept of a workflow to single-particle analysis of cryo-electron microscopy (cryo-EM) images in the RELION program. In this approach, the structure determination process is considered as a graph, where intermediate results in the form of images or metadata are the vertices, and different functionalities of the program are the edges. The new implementation automatically logs all user actions, facilitates file management and disk cleaning, and allows convenient browsing of a project's history. Moreover, new functionality to iteratively execute consecutive jobs allows on-the-fly image processing, which will lead to more efficient data acquisition by providing faster feedback on data quality. The possibility to exchange data processing procedures among users will contribute to the development of standardised image processing procedures, and hence increase accessibility for new users in this rapidly expanding field.

Download data

  • Downloaded 2,570 times
  • Download rankings, all-time:
    • Site-wide: 5,364
    • In biophysics: 93
  • Year to date:
    • Site-wide: 47,892
  • Since beginning of last month:
    • Site-wide: 49,535

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide