Rxivist logo

CTCF and Cohesin Regulate Chromatin Loop Stability with Distinct Dynamics

By Anders S. Hansen, Iryna Pustova, Claudia Cattoglio, Robert Tjian, Xavier Darzacq

Posted 12 Dec 2016
bioRxiv DOI: 10.1101/093476 (published DOI: 10.7554/eLife.25776)

Folding of mammalian genomes into spatial domains is critical for gene regulation. CTCF and cohesin control domain location by folding domains into loop structures, which are thought to be highly stable. Combining genomic, biochemical and single-molecule imaging approaches, we show that although CTCF and cohesin can physically interact, CTCF binds chromatin much more dynamically than cohesin (~1 min vs. ~22 min residence time). Moreover, after unbinding, CTCF quickly rebinds another cognate site unlike cohesin (~1 min vs. ~33 min). Thus, CTCF and cohesin form a rapidly exchanging "dynamic complex" rather than a typical stable complex. Since CTCF and cohesin are required for loop domain formation, our results suggest that chromatin loops constantly break and reform throughout the cell cycle.

Download data

  • Downloaded 2,572 times
  • Download rankings, all-time:
    • Site-wide: 2,490 out of 92,185
    • In biophysics: 63 out of 4,009
  • Year to date:
    • Site-wide: 61,615 out of 92,185
  • Since beginning of last month:
    • Site-wide: 61,010 out of 92,185

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News