Rxivist logo

The Condensin Complex Is A Mechanochemical Motor That Translocates Along DNA

By Tsuyoshi Terekawa, Shveta Bisht, Jorine M. Eeftens, Cees Dekker, Christian H. Haering, Eric C. Greene

Posted 13 May 2017
bioRxiv DOI: 10.1101/137711 (published DOI: 10.1126/science.aan6516)

Condensin plays crucial roles in chromosome organization and compaction, but the mechanistic basis for its functions remains obscure. Here, we use single-molecule imaging to demonstrate that Saccharomyces cerevisiae condensin is a molecular motor capable of ATP hydrolysis-dependent translocation along double-stranded DNA. Condensins translocation activity is rapid and highly processive, with individual complexes traveling an average distance of 10 kilobases at a velocity of ~60 base pairs per second. Our results suggest that condensin may take steps comparable in length to its ~50-nanometer coiled-coil subunits, suggestive of a translocation mechanism that is distinct from any reported DNA motor protein. The finding that condensin is a mechanochemical motor has important implications for understanding the mechanisms of chromosome organization and condensation.

Download data

  • Downloaded 2,205 times
  • Download rankings, all-time:
    • Site-wide: 3,221 out of 92,034
    • In biophysics: 84 out of 4,007
  • Year to date:
    • Site-wide: 84,241 out of 92,034
  • Since beginning of last month:
    • Site-wide: 60,057 out of 92,034

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News