Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 65,227 bioRxiv papers from 288,972 authors.

Achieving Better Than 3 Å Resolution By Single Particle Cryo-EM At 200 keV

By Mark A Herzik, Mengyu Wu, Gabriel C Lander

Posted 25 May 2017
bioRxiv DOI: 10.1101/141994 (published DOI: 10.1038/nmeth.4461)

Technical and methodological advances in single-particle cryo-electron microscopy (cryo-EM) have expanded the technique into a resolution regime that was previously only attainable by X-ray crystallography. Although single-particle cryo-EM has proven to be a useful technique for determining the structures of biomedically relevant molecules at near-atomic resolution, nearly 98% of the structures resolved to better than 4 Å resolution have been determined using 300 keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain cryo-EM reconstructions of macromolecular complexes at a range of sizes to better than 3 Å resolution using a 200 keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules, features previously thought only to be resolvable using TEMs operating at 300 keV.

Download data

  • Downloaded 5,669 times
  • Download rankings, all-time:
    • Site-wide: 347 out of 65,227
    • In biophysics: 7 out of 2,751
  • Year to date:
    • Site-wide: 1,549 out of 65,227
  • Since beginning of last month:
    • Site-wide: 8,412 out of 65,227

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)