Rxivist logo

A novel domain assembly routine for creating full-length models of membrane proteins from known domain structures

By Julia Koehler Leman, Richard Bonneau

Posted 27 Oct 2017
bioRxiv DOI: 10.1101/209213 (published DOI: 10.1021/acs.biochem.7b00995)

Membrane proteins composed of soluble and membrane domains are often studied one domain at a time. However, to understand the biological function of entire protein systems and their interactions with each other and drugs, knowledge of full-length structures or models is required. Although few computational methods exist that could potentially be used to model full-length constructs of membrane proteins, none of these methods are perfectly suited for the problem at hand. Existing methods either require an interface or knowledge of the relative orientations of the domains, are not designed for domain assembly, and none of them are developed for membrane proteins. Here we describe the first domain assembly protocol specifically designed for membrane proteins that assembles intra- and extracellular soluble domains and the transmembrane domain into models of the full-length membrane protein. Our protocol does not require an interface between the domains and samples possible domain orientations based on backbone dihedrals in the flexible linker regions, created via fragment insertion, while keeping the transmembrane domain fixed in the membrane. Our method, mp_domain_assembly, implemented in RosettaMP samples domain orientations close to the native structure and is best used in conjunction with experimental data to reduce the conformational search space.

Download data

  • Downloaded 244 times
  • Download rankings, all-time:
    • Site-wide: 58,732 out of 85,215
    • In biophysics: 2,511 out of 3,671
  • Year to date:
    • Site-wide: 80,577 out of 85,215
  • Since beginning of last month:
    • Site-wide: 80,194 out of 85,215

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)