A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein
Ahmed S. Abdelfattah,
Rosana S. Molina,
Matthew D. Wiens,
Thomas E. Hughes,
Douglas S. Kim,
Eric R. Schreiter,
Robert E. Campbell
Posted 02 Nov 2017
bioRxiv DOI: 10.1101/213082 (published DOI: 10.1186/s12915-018-0480-0)
Posted 02 Nov 2017
Genetically-encoded calcium ion (Ca2+) indicators (GECIs) are indispensable tools for measuring Ca2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs enable multicolor visualization with blue or cyan-excitable fluorophores and combined use with blue or cyan-excitable optogenetic actuators. Here we report the development, structure, and validation of a new red fluorescent Ca2+ indicator, K-GECO1, based on a circularly permutated RFP derived from the sea anemone Entacmaea quadricolor. We characterized the performance of K-GECO1 in cultured HeLa cells, dissociated neurons, stem cell derived cardiomyocytes, organotypic brain slices, zebrafish spinal cord in vivo, and mouse brain in vivo.
- Downloaded 1,275 times
- Download rankings, all-time:
- Site-wide: 11,872 out of 118,373
- In biophysics: 292 out of 4,611
- Year to date:
- Site-wide: 64,258 out of 118,373
- Since beginning of last month:
- Site-wide: 79,919 out of 118,373
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!