Rxivist logo

New routes for PTP1B allosteric inhibition by multitemperature crystallography, fragment screening and covalent tethering

By Daniel A. Keedy, Zachary B Hill, Justin T Biel, Emily Kang, T. Justin Rettenmaier, Jose Brandao-Neto, Nicholas M. Pearce, Frank von Delft, James A Wells, James S Fraser

Posted 13 Nov 2017
bioRxiv DOI: 10.1101/218966 (published DOI: 10.7554/elife.36307)

Allostery is an inherent feature of proteins and provides alternative routes to regulating function. Small-molecule allosteric inhibitors are often desirable; however, it remains challenging to identify surface sites in proteins which can bind small molecules and modulate function. We identified new allosteric sites in protein tyrosine phosphatase 1B (PTP1B) by combining multiple-temperature X-ray crystallography experiments and structure determination from hundreds of individual small-molecule fragment soaks. New modeling approaches reveal "hidden" low-occupancy conformational states for protein and ligands. Our results converge on a new allosteric site that is conformationally coupled to the active-site WPD loop, a hotspot for fragment binding, not conserved in the closest homolog, and distinct from other recently reported allosteric sites in PTP1B. Targeting this site with covalently tethered molecules allosterically inhibits enzyme activity. Overall, this work demonstrates how the ensemble nature of macromolecular structure revealed by multitemperature crystallography can be exploited for developing allosteric modulators.

Download data

  • Downloaded 726 times
  • Download rankings, all-time:
    • Site-wide: 20,149 out of 94,912
    • In biophysics: 696 out of 4,144
  • Year to date:
    • Site-wide: 55,772 out of 94,912
  • Since beginning of last month:
    • Site-wide: 44,861 out of 94,912

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News