Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 73,470 bioRxiv papers from 319,827 authors.

Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy

By Antonino Ingargiola, Maya Segal, A. Gulinatti, I. Rech, Ivan Labanca, Piera Maccagnani, M. Ghioni, Shimon Weiss, X. Michalet

Posted 21 Oct 2017
bioRxiv DOI: 10.1101/207118 (published DOI: 10.1016/j.nima.2017.11.070)

Single-molecule fluorescence spectroscopy (SMFS), based on the detection of individual molecules freely diffusing through the excitation spot of a confocal microscope, has allowed unprecedented insights into biological processes at the molecular level, but suffers from limited throughput. We have recently introduced a multispot version of SMFS, which allows achieving high-throughput SMFS by virtue of parallelization, and relies on custom silicon single-photon avalanche diode (SPAD) detector arrays. Here, we examine the premise of this parallelization approach, which is that data acquired from different spots is uncorrelated. In particular, we measure the optical crosstalk characteristics of the two 48-SPAD arrays used in our recent SMFS studies, and demonstrate that it is negligible (crosstalk probability ≤1.1 10-3) and is undetectable in cross-correlation analysis of actual single-molecule fluorescence data.

Download data

  • Downloaded 648 times
  • Download rankings, all-time:
    • Site-wide: 16,838 out of 73,470
    • In biophysics: 569 out of 3,120
  • Year to date:
    • Site-wide: 42,690 out of 73,470
  • Since beginning of last month:
    • Site-wide: 42,690 out of 73,470

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News