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Multiplex single-molecule DNA barcoding using an oligonucleotide ligation assay

By Ivo Severins, Malwina Szczepaniak, Chirlmin Joo

Posted 13 Feb 2018
bioRxiv DOI: 10.1101/265215 (published DOI: 10.1016/j.bpj.2018.08.013)

Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay (OLA) with multicolor single-molecule fluorescence. We demonstrated that 7-nt long DNA barcodes have the optimal short length to ascertain specificity while being long enough for sufficient ligation. Using four spectrally separated fluorophores to label DNA barcodes, we simultaneously distinguished four DNA target sequences differing by only a single nucleotide. Our new single-molecule approach will allow for accurate identification of low abundance molecules without the need for target DNA pre-amplification.

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