Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 60,222 bioRxiv papers from 267,718 authors.

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

By Takanori Nakane, Dari Kimanius, Erik Lindahl, Sjors H.W. Scheres

Posted 22 Mar 2018
bioRxiv DOI: 10.1101/286856 (published DOI: 10.7554/elife.36861)

Macromolecular complexes that exhibit continuous forms of structural flexibility pose a challenge for many existing tools in cryo-EM single-particle analysis. We describe a new tool, called multi-body refinement, which models flexible complexes as a user-defined number of rigid bodies that move independently from each other. Using separate focused refinements with iteratively improved partial signal subtraction, the new tool generates improved reconstructions for each of the defined bodies in a fully automated manner. Moreover, using principal component analysis on the relative orientations of the bodies over all particles in the data set, we generate movies that describe the most important motions in the data. Our results on two test cases, a cytoplasmic ribosome from Plasmodium falciparum, and the spliceosomal B-complex from yeast, illustrate how multi-body refinement can be useful to gain unique insights into the structure and dynamics of large and flexible macromolecular complexes.

Download data

  • Downloaded 3,153 times
  • Download rankings, all-time:
    • Site-wide: 947 out of 60,222
    • In biophysics: 20 out of 2,550
  • Year to date:
    • Site-wide: 2,331 out of 60,222
  • Since beginning of last month:
    • Site-wide: 4,738 out of 60,222

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News