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Massively parallel clonal analysis using CRISPR/Cas9 induced genetic scars

By Jan Philipp Junker, Bastiaan Spanjaard, Josi Peterson-Maduro, Anna Alemany, Bo Hu, Maria Florescu, Alexander van Oudenaarden

Posted 01 Jun 2016
bioRxiv DOI: 10.1101/056499

A key goal of developmental biology is to understand how a single cell transforms into a full-grown organism consisting of many cells. Although impressive progress has been made in lineage tracing using imaging approaches, analysis of vertebrate lineage trees has mostly been limited to relatively small subsets of cells. Here we present scartrace, a strategy for massively parallel clonal analysis based on Cas9 induced genetic scars in the zebrafish.

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