Rxivist logo

Massively parallel single cell lineage tracing using CRISPR/Cas9 induced genetic scars

By Bastiaan Spanjaard, Bo Hu, Nina Mitic, Jan Philipp Junker

Posted 19 Oct 2017
bioRxiv DOI: 10.1101/205971 (published DOI: 10.1038/nbt.4124)

A key goal of developmental biology is to understand how a single cell transforms into a full-grown organism consisting of many different cell types. Single-cell RNA- sequencing (scRNA-seq) has become a widely-used method due to its ability to identify all cell types in a tissue or organ in a systematic manner. However, a major challenge is to organize the resulting taxonomy of cell types into lineage trees revealing the developmental origin of cells. Here, we present a strategy for simultaneous lineage tracing and transcriptome profiling in thousands of single cells. By combining scRNA-seq with computational analysis of lineage barcodes generated by genome editing of transgenic reporter genes, we reconstruct developmental lineage trees in zebrafish larvae and adult fish. In future analyses, LINNAEUS (LINeage tracing by Nuclease-Activated Editing of Ubiquitous Sequences) can be used as a systematic approach for identifying the lineage origin of novel cell types, or of known cell types under different conditions.

Download data

  • Downloaded 2,482 times
  • Download rankings, all-time:
    • Site-wide: 2,515 out of 88,268
    • In systems biology: 65 out of 2,297
  • Year to date:
    • Site-wide: 26,911 out of 88,268
  • Since beginning of last month:
    • Site-wide: 44,727 out of 88,268

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News