Rxivist logo

Transcriptional burst initiation and polymerase pause release are key control points of transcriptional regulation

By Caroline Bartman, Cheryl A. Keller, Belinda Giardine, Ross C. Hardison, Gerd A. Blobel, Arjun Raj

Posted 02 Mar 2018
bioRxiv DOI: 10.1101/275354 (published DOI: 10.1016/j.molcel.2018.11.004)

Transcriptional regulation occurs via changes to the rates of various biochemical processes. Sequencing based approaches that average together many cells have suggested that polymerase binding and polymerase release from promoter proximal pausing are two key regulated steps in the transcriptional process. However, single cell studies have revealed that transcription occurs in short, discontinuous bursts, suggesting that transcriptional burst initiation and termination might also be regulated steps. Here, we develop and apply a quantitative framework to connect changes in both Pol II ChIP sequencing and single cell transcriptional measurements to changes in the rates of specific steps of transcription. Using a number of global and targeted transcriptional regulatory perturbations, we show that burst initiation rate is indeed a key regulated step, demonstrating that transcriptional activity can be frequency modulated. Polymerase pause release is a second key regulated step, but the rate of polymerase binding is not changed by any of the biological perturbations we examined. Our results establish an important role for transcriptional burst regulation in the control of gene expression.

Download data

  • Downloaded 3,811 times
  • Download rankings, all-time:
    • Site-wide: 1,412 out of 101,463
    • In systems biology: 32 out of 2,575
  • Year to date:
    • Site-wide: 22,127 out of 101,463
  • Since beginning of last month:
    • Site-wide: 64,477 out of 101,463

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News

  • 20 Oct 2020: Support for sorting preprints using Twitter activity has been removed, at least temporarily, until a new source of social media activity data becomes available.
  • 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
  • 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
  • 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
  • 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
  • 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
  • 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
  • 22 Jan 2019: Nature just published an article about Rxivist and our data.
  • 13 Jan 2019: The Rxivist preprint is live!