Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,524 bioRxiv papers from 264,866 authors.
Assembly of Radically Recoded E. coli Genome Segments
Julie E. Norville,
Cameron L. Gardner,
Conor K. Camplisson,
David K. Barclay,
Katerina A. Turner,
James E. DiCarlo,
Christopher J. Gregg,
Barry L. Wanner,
George M Church
Posted 19 Aug 2016
bioRxiv DOI: 10.1101/070417
Posted 19 Aug 2016
The large potential of radically recoded organisms (RROs) in medicine and industry depends on improved technologies for efficient assembly and testing of recoded genomes for biosafety and functionality. Here we describe a next generation platform for conjugative assembly genome engineering, termed CAGE 2.0, that enables the scarless integration of large synthetically recoded E. coli segments at isogenic and adjacent genomic loci. A stable tdk dual selective marker is employed to facilitate cyclical assembly and removal of attachment sites used for targeted segment delivery by site-specific recombination. Bypassing the need for vector transformation harnesses the multi Mb capacity of CAGE, while minimizing artifacts associated with RecA-mediated homologous recombination. Our method expands the genome engineering toolkit for radical modification across many organisms and recombinase-mediated cassette exchange (RMCE).
- Downloaded 2,330 times
- Download rankings, all-time:
- Site-wide: 1,573 out of 57,524
- In synthetic biology: 29 out of 551
- Year to date:
- Site-wide: 15,310 out of 57,524
- Since beginning of last month:
- Site-wide: 22,820 out of 57,524
Downloads over time
Distribution of downloads per paper, site-wide
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!