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The Sequence of 1504 Mutants in the Model Rice Variety Kitaake Facilitates Rapid Functional Genomic Studies

By Guotian Li, Rashmi Jain, Mawsheng Chern, Nikki T Pham, Joel A Martin, Tong Wei, Wendy S Schackwitz, Anna M Lipzen, Phat Q Duong, Kyle C Jones, Liangrong Jiang, Deling Ruan, Diane Bauer, Yi Peng, Kerrie W Barry, Jeremy Schmutz, Pamela C. Ronald

Posted 23 Feb 2017
bioRxiv DOI: 10.1101/111237 (published DOI: 10.1105/tpc.17.00154)

The availability of a whole-genome sequenced mutant population and the cataloging of mutations of each line at a single-nucleotide resolution facilitates functional genomic analysis. To this end, we generated and sequenced a fast-neutron-induced mutant population in the model rice cultivar Kitaake (Oryza sativa L. ssp. japonica), which completes its life cycle in 9 weeks. We sequenced 1,504 mutant lines at 45-fold coverage and identified 91,513 mutations affecting 32,307 genes, 58% of all rice genes. We detected an average of 61 mutations per line. Mutation types include single base substitutions, deletions, insertions, inversions, translocations, and tandem duplications. We observed a high proportion of loss-of-function mutations. Using this mutant population, we identified an inversion affecting a single gene as the causative mutation for the short-grain phenotype in one mutant line with a small segregating population. This result reveals the usefulness of the resource for efficient identification of genes conferring specific phenotypes. To facilitate public access to this genetic resource, we established an open access database called KitBase that provides access to sequence data and seed stocks, enabling rapid functional genomic studies of rice.

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