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A bacterial GW-effector directly targets Arabidopsis Argonaute 1 to suppress PAMP-triggered immunity and cause disease

By Odon Thiebeauld, Magali Charvin, Meenu Singla Rastogi, Fan Yang, Dominique Pontier, Cecile Pouzet, Laure Bapaume, Delase Amsefe, Guangyong Li, Laurent Deslandes, Thierry Lagrange, James R. Alfano, Lionel Navarro

Posted 07 Nov 2017
bioRxiv DOI: 10.1101/215590

Pseudomonas syringae (P. syringae) type-III effectors were previously found to suppress the Arabidopsis microRNA (miRNA) pathway through unknown mechanisms. Here, we first show that the P. syringae HopT1-1 effector promotes pathogenicity by suppressing the Arabidopsis Argonaute 1 (AGO1)-dependent miRNA pathway. We further demonstrate that HopT1-1 physically interacts with Arabidopsis AGO1 through conserved glycine/tryptophan (GW) motifs. Importantly, this AGO-binding platform was found to be essential for the ability of HopT1-1 to suppress both miRNA activity and PAMP-Triggered Immunity (PTI). These results therefore indicate that the RNA silencing suppression activity of HopT1-1 is intimately coupled to its virulence function. Overall, these findings provide the first evidence that a bacterial effector has evolved to directly target an AGO protein to suppress PTI and cause disease.

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