Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,789 bioRxiv papers from 265,997 authors.

The MoT3 assay does not distinguish between Magnaporthe oryzae wheat and rice blast isolates from Bangladesh

By Dipali Gupta, Claudia Sarai Reyes Avila, Joe Win, Darren Soanes, Lauren S. Ryder, Daniel Croll, Pallab Bhattacharjee, Shaid Hossain, Nur Uddin Mahmud, Md. Shabab Mehbub, Musrat Zahan Surovy, Nicholas Talbot, Sophien Kamoun, Tofazzal Islam

Posted 14 Jun 2018
bioRxiv DOI: 10.1101/345215 (published DOI: 10.1094/PHYTO-06-18-0199-LE)

The blast fungus Magnaporthe oryzae is comprised of lineages that exhibit varying degrees of specificity on about 50 grass hosts, including rice, wheat and barley. Reliable diagnostic tools are essential given that the pathogen has a propensity to jump to new hosts and spread to new geographic regions. Of particular concern is wheat blast, which has suddenly appeared in Bangladesh in 2016 before spreading to neighboring India. In these Asian countries, wheat blast strains are now co-occurring with the destructive rice blast pathogen raising the possibility of genetic exchange between these destructive pathogens. We assessed the recently described MoT3 diagnostic assay and found that it did not distinguish between wheat and rice blast isolates from Bangladesh. The assay is based on primers matching the WB12 sequence corresponding to a fragment of the M. oryzae MGG_02337 gene annotated as a short chain dehydrogenase. These primers could not reliably distinguish between wheat and rice blast isolates from Bangladesh based on DNA amplification experiments performed in separate laboratories in Bangladesh and in the UK. In addition, comparative genomics of the WB12 sequence revealed a complex underlying genetic structure with related sequences across M. oryzae strains and in both rice and wheat blast isolates. We, therefore, caution against the indiscriminate use of this assay to identify wheat blast.

Download data

  • Downloaded 523 times
  • Download rankings, all-time:
    • Site-wide: 16,661 out of 57,789
    • In plant biology: 408 out of 1,599
  • Year to date:
    • Site-wide: 27,181 out of 57,789
  • Since beginning of last month:
    • Site-wide: 22,798 out of 57,789

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News