Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 73,536 bioRxiv papers from 320,015 authors.

Sub-tomogram averaging in RELION

By Tanmay A.M. Bharat, Sjors H.W. Scheres

Posted 04 Nov 2015
bioRxiv DOI: 10.1101/030544 (published DOI: 10.1038/nprot.2016.124)

Electron cryo-tomography (cryo-ET) and sub-tomogram averaging allow structure determination of macromolecules in situ, and are gaining in popularity for initial model generation for single- particle analysis. We describe herein, a protocol for sub-tomogram averaging from cryo-ET data using the RELION software. We describe how to calculate newly developed three-dimensional models for the contrast transfer function and the missing wedge of each sub-tomogram, and how to use these models for regularized-likelihood refinement. This approach has been implemented in the existing workflow for single-particle analysis, so that users may conveniently tap into existing capabilities of the RELION software. As example applications, we present analyses of purified hepatitis B capsid particles and S. cerevisiae 80S ribosomes. In both cases, we show that following initial classification, sub-tomogram averaging in RELION allows de novo generation of initial models, and provides high-resolution maps where secondary structure elements are resolved.

Download data

  • Downloaded 2,739 times
  • Download rankings, all-time:
    • Site-wide: 1,547 out of 73,536
    • In molecular biology: 38 out of 2,394
  • Year to date:
    • Site-wide: 12,422 out of 73,536
  • Since beginning of last month:
    • Site-wide: 12,422 out of 73,536

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News