Rxivist logo

Sub-tomogram averaging in RELION

By Tanmay A.M. Bharat, Sjors H.W. Scheres

Posted 04 Nov 2015
bioRxiv DOI: 10.1101/030544 (published DOI: 10.1038/nprot.2016.124)

Electron cryo-tomography (cryo-ET) and sub-tomogram averaging allow structure determination of macromolecules in situ, and are gaining in popularity for initial model generation for single- particle analysis. We describe herein, a protocol for sub-tomogram averaging from cryo-ET data using the RELION software. We describe how to calculate newly developed three-dimensional models for the contrast transfer function and the missing wedge of each sub-tomogram, and how to use these models for regularized-likelihood refinement. This approach has been implemented in the existing workflow for single-particle analysis, so that users may conveniently tap into existing capabilities of the RELION software. As example applications, we present analyses of purified hepatitis B capsid particles and S. cerevisiae 80S ribosomes. In both cases, we show that following initial classification, sub-tomogram averaging in RELION allows de novo generation of initial models, and provides high-resolution maps where secondary structure elements are resolved.

Download data

  • Downloaded 3,139 times
  • Download rankings, all-time:
    • Site-wide: 1,708 out of 89,568
    • In molecular biology: 49 out of 3,037
  • Year to date:
    • Site-wide: 4,794 out of 89,568
  • Since beginning of last month:
    • Site-wide: 8,153 out of 89,568

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News