Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,853 bioRxiv papers from 309,208 authors.

A Dynamic Mode of Mitotic Bookmarking by Transcription Factors

By Sheila S. Teves, Luye An, Anders S. Hansen, Liangqi Xie, Xavier Darzacq, Robert Tjian

Posted 28 Jul 2016
bioRxiv DOI: 10.1101/066464 (published DOI: 10.7554/eLife.22280)

During mitosis, transcription is shut off, chromatin condenses, and most transcription factors (TFs) are reported to be excluded from chromosomes. How do daughter cells re-establish the original transcription program? Recent discoveries that a select set of TFs remain bound on mitotic chromosomes suggest a potential mechanism for maintaining transcriptional programs through the cell cycle termed mitotic bookmarking. Here we report instead that many TFs remain associated with chromosomes, and that the exclusion previously described is largely a fixation artifact. In particular, most TFs we tested are significantly enriched on mitotic chromosomes. Studies with Sox2 reveal that this mitotic interaction is more dynamic than in interphase and requires both DNA binding and nuclear import. Furthermore, this dynamic mode results from lack of transcriptional activation rather than decreased accessibility of underlying DNA sequences in mitosis. The nature of the cross-linking artifact prompts careful re-examination of the role of TFs in mitotic bookmarking.

Download data

  • Downloaded 2,091 times
  • Download rankings, all-time:
    • Site-wide: 2,389 out of 70,853
    • In molecular biology: 64 out of 2,272
  • Year to date:
    • Site-wide: 25,898 out of 70,853
  • Since beginning of last month:
    • Site-wide: 50,899 out of 70,853

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News