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Spotiton: New Features and Applications

By Venkata P Dandey, Hui Wei, Zhening Zhang, Yong Zi Tan, Priyamvada Acharya, Edward T. Eng, William J. Rice, Peter A Kahn, Clinton S. Potter, Bridget Carragher

Posted 08 Dec 2017
bioRxiv DOI: 10.1101/230151 (published DOI: 10.1016/j.jsb.2018.01.002)

We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ~0.3nm resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.

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