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Organ-specific translation elongation rates measured by in vivo ribosome profiling

By Maxim V. Gerashchenko, Zalan Peterfi, Vadim N. Gladyshev

Posted 08 Mar 2018
bioRxiv DOI: 10.1101/279257

Protein synthesis and degradation are intricate biological processes involving more than a hundred proteins operating in a highly orchestrated fashion. Despite the progress, few options are available to access translation in live animals as the increase in animal's complexity limits the repertoire of experimental tools that could be applied to observe and manipulate processes within animal's body, organs, and individual cells. It this study, we developed a labeling-free method for measuring organ- and cell-type specific translation elongation rates. It is based on a time-resolved delivery of translation initiation and elongation inhibitors in live animals followed by ribosome profiling. It also reports translation initiation sites in an organ-specific manner. Using this method, we found that the elongation rates differ among mouse organs and determined them to be 6.8, 5.2, and 4.4 amino acids per sec for liver, kidney, and skeletal muscle, respectively.

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