Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,793 bioRxiv papers from 266,023 authors.

Telomere-loop dynamics in chromosome end protection

By David Van Ly, Ronnie Ren Jie Low, Sonja Frölich, Tara K Bartolec, Georgia R. Kafer, Hilda A. Pickett, Katharina Gaus, Anthony J Cesare

Posted 09 Mar 2018
bioRxiv DOI: 10.1101/279877 (published DOI: 10.1016/j.molcel.2018.06.025)

We used super-resolution microscopy to investigate the role of macromolecular telomere structure in chromosome end protection. In murine and human cells with reduced TRF2, we find that ATM-activation at chromosome ends occurs with a structural change from t-loops to linearized chromosome ends through t-loop unfolding. Comparably, we find Aurora B kinase regulates telomere linearity concurrent with ATM activation at telomeres during mitotic arrest. Using a separation of function allele, we find that the TRFH domain of TRF2 regulates t-loop formation while suppressing ATM activity. Notably, we demonstrate that telomere linearity and ATM activation occur separately from telomere fusion via non-homologous end-joining (NHEJ). Further, we show that linear DDR-positive telomeres can remain resistant to fusion, even during an extended G1-arrest when NHEJ is most active. Collectively, these results suggest t-loops act as conformational switches that regulate ATM activation at chromosome ends independent of mechanisms to suppress chromosome end fusion.

Download data

  • Downloaded 1,110 times
  • Download rankings, all-time:
    • Site-wide: 5,485 out of 57,793
    • In molecular biology: 158 out of 1,772
  • Year to date:
    • Site-wide: 19,525 out of 57,793
  • Since beginning of last month:
    • Site-wide: 34,541 out of 57,793

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)