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VCPA: genomic variant calling pipeline and data management tool for Alzheimer's Disease Sequencing Project

By Yuk Yee Leung, Otto Valladares, Yi-Fan Chou, Han-Jen Lin, Amanda B Kuzma, Laura Cantwell, Liming Qu, Prabhakaran Gangadharan, Alzheimer’s Disease Sequencing Project (ADSP), William J Salerno, Gerard D. Schellenberg, Li-San Wang

Posted 21 May 2018
bioRxiv DOI: 10.1101/327395 (published DOI: 10.1093/bioinformatics/bty894)

Summary: We report VCPA, our SNP/Indel Variant Calling Pipeline and data management tool used for analysis of whole genome and exome sequencing (WGS/WES) for the Alzheimer's Disease Sequencing Project. VCPA consists of two independent but linkable components: pipeline and tracking database. The pipeline is coded in Workflow Description Language and is fully optimized for the Amazon elastic compute cloud environment. This includes steps for processing raw sequence reads including read alignment, and all the way up to variant calling using GATK. The tracking database allows users to dynamically view the statuses of jobs running and the quality metrics reported by the pipeline. Users can thus monitor the production process and diagnose if any problem arises during the procedure. All quality metrics (>100 collected per processed genome) are stored in the database, thus facilitating users to compare, share and visualize the results. To summarize, VCPA is functional equivalent to the CCDG/TOPMed pipeline. Together with the dockerized database (also available as Amazon Machine Image), users can easily process any WGS/WES data on Amazon cloud with minimal installation. Availability: VCPA is released under the MIT license and is available for academic and nonprofit use for free. The pipeline source code and step-by-step instructions are available from the National Institute on Aging Genetics of Alzheimer's Disease Data Storage Site (http://www.niagads.org/VCPA).

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