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AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design

By Kathleen Keough, Svetlana Lyalina, Michael P Olvera, Sean Whalen, Bruce R Conklin, Katherine Pollard

Posted 08 Jun 2018
bioRxiv DOI: 10.1101/342923 (published DOI: 10.1186/s13059-019-1783-3)

The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. However, current tools only design guides (sgRNAs) for a reference genome, not taking into account individual variants which may generate, remove, or modify CRISPR/Cas sgRNA sites. Here we describe ExcisionFinder, a tool for designing personalized and allele-specific sgRNAs for genome editing. We leverage >2,500 human genomes to identify optimized pairs of sgRNAs that can be used for human therapeutic editing in large populations in the future.

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