Rxivist logo

Characterizing the fitness landscape, a representation of fitness for a large set of genotypes is key to understanding how genetic information is interpreted to create functional organisms. Here we determined the evolutionarily-relevant segment of the fitness landscape of His3, a gene coding for an enzyme in the histidine synthesis pathway, focusing on combinations of amino acid states found at orthologous sites of extant species. The His3 fitness landscape is dominated by synergistic epistasis. For 80% of substitutions, the effect on fitness depended on the genetic background. Furthermore, at 67% of sites, substitutions are under sign epistasis, having both strongly positive and negative effects in different genetic backgrounds. 46% of sites were under reciprocal sign epistasis. Sign epistasis affected few genotypes but involved the interaction of multiple sites which makes multidimensionality of the His3 fitness landscape essential, with the number of dimensions of the landscape approximately one quarter of the sequence length.

Download data

  • Downloaded 1,320 times
  • Download rankings, all-time:
    • Site-wide: 7,303 out of 89,715
    • In evolutionary biology: 367 out of 5,480
  • Year to date:
    • Site-wide: 19,965 out of 89,715
  • Since beginning of last month:
    • Site-wide: 22,714 out of 89,715

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)