Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 57,789 bioRxiv papers from 265,997 authors.

Cell Dynamics Underlying Oriented Growth Of The Drosophila Imaginal Wing Disc

By Natalie A. Dye, Marko Popović, Stephanie Spannl, Raphaël Etournay, Dagmar Kainmüller, Eugene W. Myers, Frank Jülicher, Suzanne Eaton

Posted 19 May 2017
bioRxiv DOI: 10.1101/140038 (published DOI: 10.1242/dev.155069)

Quantitative analysis of the dynamic cellular mechanisms shaping the Drosophila wing during its larval growth phase has been limited, impeding our ability to understand how morphogen patterns regulate tissue shape. Such analysis requires imaging explants under conditions that maintain both growth and patterning, as well as methods to quantify how much cellular behaviors change tissue shape. Here, we demonstrate a key requirement for the steroid hormone 20-hydroxyecdysone (20E) in the maintenance of numerous patterning systems in vivo and in explant culture. We find that low concentrations of 20E support prolonged proliferation in explanted wing discs in the absence of insulin, incidentally providing novel insight into the hormonal regulation of imaginal growth. We use 20E-containing media to directly observe growth and apply recently developed methods for quantitatively decomposing tissue shape changes into cellular contributions. We discover that while cell divisions drive tissue expansion along one axis, their contribution to expansion along the orthogonal axis is canceled by cell rearrangements and cell shape changes. This finding raises the possibility that anisotropic mechanical constraints contribute to growth orientation in the wing disc.

Download data

  • Downloaded 791 times
  • Download rankings, all-time:
    • Site-wide: 9,415 out of 57,789
    • In developmental biology: 210 out of 1,724
  • Year to date:
    • Site-wide: 34,690 out of 57,789
  • Since beginning of last month:
    • Site-wide: 17,990 out of 57,789

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)