Rxivist logo

An FGF-driven feed-forward circuit patterns the cardiopharyngeal mesoderm in space and time

By Florian Razy-Krajka, Basile Gravez, Nicole Kaplan, Claudia Racioppi, Wei Wang, Lionel Christiaen

Posted 17 May 2017
bioRxiv DOI: 10.1101/138701 (published DOI: 10.7554/elife.29656)

In embryos, multipotent progenitors divide to produce distinct progeny and express their full potential. In vertebrates, multipotent cardiopharyngeal progenitors produce second-heart-field-derived cardiomyocytes, and branchiomeric skeletal head muscles. However, the mechanisms underlying these early fate choices remain largely elusive. The tunicate Ciona emerged as an attractive model to study early cardiopharyngeal development at high resolution: through two asymmetric and oriented divisions, defined cardiopharyngeal progenitors produce distinct first and second heart precursors, and pharyngeal muscle (aka atrial siphon muscle, ASM) precursors. Here, we demonstrate that differential FGF-MAPK signaling distinguishes between heart and ASM precursors. We characterize a feed-forward circuit that promotes the successive activations of essential ASM determinants, Hand-related, Tbx1/10 and Ebf. Finally, we show that coupling FGF-MAPK restriction and cardiopharyngeal network deployment with cell divisions defines the timing of gene expression and permits the emergence of diverse cell types from multipotent progenitors.

Download data

  • Downloaded 656 times
  • Download rankings, all-time:
    • Site-wide: 21,662 out of 88,741
    • In developmental biology: 546 out of 2,648
  • Year to date:
    • Site-wide: 59,340 out of 88,741
  • Since beginning of last month:
    • Site-wide: 33,661 out of 88,741

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News