An unconventional myosin, myosin 1d regulates Kupffer's vesicle morphogenesis and laterality
Madeline J. Clark,
Donna B. Stolz,
Simon C. Watkins,
Jeffrey D. Amack,
Cecilia W. Lo,
Posted 26 Feb 2018
bioRxiv DOI: 10.1101/268789 (published DOI: 10.1038/s41467-018-05866-2)
Posted 26 Feb 2018
Establishing left-right (LR) asymmetry is a fundamental process essential for arrangement of visceral organs during development. In vertebrates, motile cilia driven fluid flow in the left-right organizer (LRO) is essential for initiating symmetry breaking event. Without a definite LRO structure in invertebrates, LR asymmetry is initiated at a cellular level by actin-myosin driven chirality. In Drosophila, myosin1D drives tissue-specific chirality in hind-gut looping. Here, we show that myosin 1d (myo1d) is essential for establishing LR asymmetry in zebrafish. Using super-resolution microscopy, we show that the zebrafish LRO, Kupffer's vesicle (KV), fails to form proper lumen size in the absence of myo1d. This process requires directed vacuolar trafficking in KV epithelial cells. Interestingly, the vacuole transporting function of zebrafish Myo1d can be substituted by myosin1C derived from an ancient eukaryote, Acanthamoeba castellanii, where it regulates the transport of contractile vacuoles. Our findings reveal an evolutionarily conserved role for an unconventional myosin in vacuole trafficking, lumen formation and determining laterality.
- Downloaded 353 times
- Download rankings, all-time:
- Site-wide: 50,500 out of 100,488
- In developmental biology: 1,484 out of 3,006
- Year to date:
- Site-wide: 87,163 out of 100,488
- Since beginning of last month:
- Site-wide: None out of 100,488
Downloads over time
Distribution of downloads per paper, site-wide
- 20 Oct 2020: Support for sorting preprints using Twitter activity has been removed, at least temporarily, until a new source of social media activity data becomes available.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!