Rxivist logo

Dynamic multifactor hubs interact transiently with sites of active transcription in Drosophila embryos

By Mustafa Mir, Michael R Stadler, Stephan A Ortiz, Melissa M. Harrison, Xavier Darzacq, Michael B. Eisen

Posted 26 Jul 2018
bioRxiv DOI: 10.1101/377812 (published DOI: 10.7554/elife.40497)

The regulation of transcription requires the coordination of numerous activities on DNA, yet it remains poorly understood how transcription factors facilitate these multiple functions. Here we use lattice light-sheet microscopy to integrate single-molecule and high-speed 4D imaging in developing Drosophila embryos to study the nuclear organization and interactions of the key patterning factors Zelda and Bicoid. In contrast to previous studies suggesting stable, cooperative binding, we show that both factors interact with DNA with surprisingly high off-rates. We find that both factors form dynamic subnuclear hubs, and that Bicoid binding is enriched within Zelda hubs. Remarkably, these hubs are both short lived and interact only transiently with sites of active Bicoid dependent transcription. Based on our observations we hypothesize that, beyond simply forming bridges between DNA and the transcription machinery, transcription factors can organize other proteins into hubs that transiently drive multiple activities at their gene targets.

Download data

  • Downloaded 1,211 times
  • Download rankings, all-time:
    • Site-wide: 8,680 out of 92,068
    • In developmental biology: 170 out of 2,731
  • Year to date:
    • Site-wide: 69,170 out of 92,068
  • Since beginning of last month:
    • Site-wide: 64,590 out of 92,068

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)