Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 65,227 bioRxiv papers from 288,972 authors.

Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system

By Jennifer K Heppert, Daniel J Dickinson, Ariel M Pani, Christopher D Higgins, Annette Steward, Julie Ahringer, Jeffrey R Kuhn, Bob Goldstein

Posted 19 Feb 2016
bioRxiv DOI: 10.1101/040279 (published DOI: 10.1091/mbc.E16-01-0063)

Fluorescent protein tags are fundamental tools used to visualize gene products and analyze their dynamics in vivo. Recent advances in genome editing have enabled precise insertion of fluorescent protein tags into the genomes of diverse organisms. These advances expand the potential of in vivo imaging experiments, and they facilitate experimentation with new, bright, photostable fluorescent proteins. Most quantitative comparisons of the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that govern their performance in cells or organisms. To address the gap we quantitatively assessed fluorescent protein properties in vivo in an animal model system. We generated transgenic C. elegans strains expressing green, yellow, or red fluorescent proteins in embryos, and we imaged embryos expressing different fluorescent proteins under the same conditions for direct comparison. We found that mNeonGreen was not bright in vivo as predicted based on in vitro data, but that mNeonGreen is a better tag than GFP for specific kinds of experiments, and we report on optimal red fluorescent proteins. These results identify ideal fluorescent proteins for imaging in vivo in C. elegans embryos, and they suggest good candidate fluorescent proteins to test in other animal model systems.

Download data

  • Downloaded 4,425 times
  • Download rankings, all-time:
    • Site-wide: 542 out of 65,227
    • In cell biology: 16 out of 3,138
  • Year to date:
    • Site-wide: 5,691 out of 65,227
  • Since beginning of last month:
    • Site-wide: 19,749 out of 65,227

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News