Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,284 bioRxiv papers from 296,196 authors.

Nanoscale Manipulation Of Membrane Curvature For Probing Endocytosis In Live Cells

By Wenting Zhao, Lindsey Hanson, Hsin-Ya Lou, Matthew Akamatsu, Praveen D. Chowdary, Francesca Santoro, Jessica R. Marks, Alexandre Grassart, David G. Drubin, Yi Cui, Bianxiao Cui

Posted 30 Mar 2017
bioRxiv DOI: 10.1101/122275 (published DOI: 10.1038/nnano.2017.98)

Clathrin-mediated endocytosis (CME) involves nanoscale bending and inward budding of the plasma membrane, by which cells regulate both the distribution of membrane proteins and the entry of extracellular species. Extensive studies have shown that CME proteins actively modulate the plasma membrane curvature. However, the reciprocal regulation of how plasma membrane curvature affects the activities of endocytic proteins is much less explored, despite studies suggesting that membrane curvature itself can trigger biochemical reactions. This gap in our understanding is largely due to technical challenges in precisely controlling the membrane curvature in live cells. In this work, we use patterned nanostructures to generate well-defined membrane curvatures ranging from +50 nm to −500 nm radius of curvature. We find that the positively curved membranes are CME hotspots, and that key CME proteins, clathrin and dynamin, show a strong preference toward positive membrane curvatures with a radius < 200 nm. Of ten CME related proteins we examined, all show preferences to positively curved membrane. By contrast, other membrane-associated proteins and non-CME endocytic protein, caveolin1, show no such curvature preference. Therefore, nanostructured substrates constitute a novel tool for investigating curvature-dependent processes in live cells.

Download data

  • Downloaded 1,143 times
  • Download rankings, all-time:
    • Site-wide: 6,244 out of 67,284
    • In cell biology: 197 out of 3,268
  • Year to date:
    • Site-wide: 45,894 out of 67,284
  • Since beginning of last month:
    • Site-wide: 50,125 out of 67,284

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News