Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,351 bioRxiv papers from 296,699 authors.
A general method to fine-tune fluorophores for live-cell and in vivo imaging
Jonathan B. Grimm,
Anand K Muthusamy,
Timothy A. Brown,
William C. Lemon,
John J Macklin,
Phillip J. Keller,
Luke D. Lavis
Posted 14 Apr 2017
bioRxiv DOI: 10.1101/127613 (published DOI: 10.1038/nmeth.4403)
Posted 14 Apr 2017
Pushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings into classic fluorophore structures elicits substantial increases in brightness and photostability, resulting in the 'Janelia Fluor' (JF) series of dyes. Here, we refine and extend this strategy, showing that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties with unprecedented precision. This strategy yields a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red with utility in live cells, tissue, and animals.
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