Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 66,916 bioRxiv papers from 294,600 authors.
Observing the Cell in Its Native State: Imaging Subcellular Dynamics in Multicellular Organisms
Daniel E Milkie,
Ian A. Swinburne,
Kishore R Mosaliganti,
Zach M Collins,
Tom W Hiscock,
Abraham Q Kohrman,
Taylor N Medwig,
Elliot M. Meyerowitz,
David G. Drubin,
Benjamin L Martin,
David Q Matus,
Sean G Megason,
Posted 08 Jan 2018
bioRxiv DOI: 10.1101/243352 (published DOI: 10.1126/science.aaq1392)
Posted 08 Jan 2018
True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution without inducing undue stress on either. We combined lattice light sheet microscopy with two-channel adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages, and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.
- Downloaded 12,834 times
- Download rankings, all-time:
- Site-wide: 73 out of 66,916
- In cell biology: 1 out of 3,241
- Year to date:
- Site-wide: 5,304 out of 66,916
- Since beginning of last month:
- Site-wide: 12,346 out of 66,916
Downloads over time
Distribution of downloads per paper, site-wide
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!