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Multiplexed imaging using same species primary antibodies with signal amplification

By Yu Wang, Wenxin Xie, Richie E. Kohman, George M Church

Posted 01 Mar 2018
bioRxiv DOI: 10.1101/274456

Multiplexed IF imaging using same species antibodies is in great demand as multiplexed IF is a general practice in biological and clinical laboratories whilst antibodies from different species are hardly available. We here presented a robust method to eliminate the use of secondary antibodies by replacing secondary antibody signal amplification with DNA-based Hybridization-Chain-Reaction signal amplification. We achieved as much as 89-fold signal enhancement, and four target imaging using all mouse primary antibodies.

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