Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,853 bioRxiv papers from 309,208 authors.
Evidence for an Integrated Gene Repression Mechanism based on mRNA Isoform Toggling in Human Cells
Juliet C Barker,
Leon Y. Chan,
Gloria A Brar
Posted 13 Feb 2018
bioRxiv DOI: 10.1101/264721 (published DOI: 10.1534/g3.118.200802)
Posted 13 Feb 2018
We recently discovered a common mode of gene regulation in budding yeast, by which mRNA production represses protein expression. Whether this regulatory mechanism is conserved was unknown. Here we find that a similar mechanism regulates the human oncogene MDM2, which is transcribed from two promoters. Transcription from the distal MDM2 promoter produces a poorly translated mRNA isoform and transcription from the proximal promoter produces a well-translated transcript. Remarkably, we find that down-regulation of transcription from the distal promoter markedly up-regulates expression from the proximal promoter and results in the loss of histone H3K36 trimethylation marks. Moreover, we observe transcript toggling between the two different MDM2 isoforms as a natural part of two distinct human embryonic stem cell differentiation programs. We conclude that the integrated gene repression mechanism recently identified in yeast is conserved in human cells.
- Downloaded 782 times
- Download rankings, all-time:
- Site-wide: 12,222 out of 70,857
- In genetics: 850 out of 3,927
- Year to date:
- Site-wide: 32,924 out of 70,857
- Since beginning of last month:
- Site-wide: 50,856 out of 70,857
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!