Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 55,243 bioRxiv papers from 254,840 authors.

A rapid and robust method for single cell chromatin accessibility profiling

By Xi Chen, Ricardo J Miragaia, Kedar Nath Natarajan, Sarah A Teichmann

Posted 27 Apr 2018
bioRxiv DOI: 10.1101/309831 (published DOI: 10.1038/s41467-018-07771-0)

The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is widely used to identify regulatory regions throughout the genome. However, very few studies have been performed at the single cell level (scATAC-seq) due to technical challenges. Here we developed a simple and robust plate-based scATAC-seq method, combining upfront bulk tagmentation with single-nuclei sorting. By profiling over 3,000 splenocytes, we identify distinct immune cell types and reveal cell type-specific regulatory regions and related transcription factors.

Download data

  • Downloaded 4,945 times
  • Download rankings, all-time:
    • Site-wide: 347 out of 55,243
    • In genomics: 91 out of 3,910
  • Year to date:
    • Site-wide: 357 out of 55,243
  • Since beginning of last month:
    • Site-wide: 1,234 out of 55,243

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)