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A rapid and robust method for single cell chromatin accessibility profiling

By Xi Chen, Ricardo J Miragaia, Kedar Nath Natarajan, Sarah A Teichmann

Posted 27 Apr 2018
bioRxiv DOI: 10.1101/309831 (published DOI: 10.1038/s41467-018-07771-0)

The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is widely used to identify regulatory regions throughout the genome. However, very few studies have been performed at the single cell level (scATAC-seq) due to technical challenges. Here we developed a simple and robust plate-based scATAC-seq method, combining upfront bulk tagmentation with single-nuclei sorting. By profiling over 3,000 splenocytes, we identify distinct immune cell types and reveal cell type-specific regulatory regions and related transcription factors.

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