A rhesus macaque model of Asia lineage Zika virus infection
Dawn M Dudley,
Andrea M Weiler,
Kim L Weisgrau,
Mariel S. Mohns,
Meghan E. Breitbach,
Mustafa N Rasheed,
Christina M. Newman,
Dane D Gellerup,
Louise H. Moncla,
Michele L. Schotkzo,
Jennifer M. Hayes,
Josh A Eudailey,
M Anthony Moody,
Sallie R. Permar,
Shelby L. O’Connor,
Eva G Rakasz,
Heather A. Simmons,
Thaddeus G. Golos,
Jorge E Osorio,
Thomas C Friedrich,
David H. O'Connor
Posted 30 Mar 2016
bioRxiv DOI: 10.1101/046334
Posted 30 Mar 2016
Infection with Asian lineage Zika virus has been associated with Guillain-Barre syndrome and fetal abnormalities 1-4, but the mechanisms and risk factors for these outcomes remain unknown. Here we show that rhesus macaques are susceptible to infection by an Asian-lineage virus closely related to strains currently circulating in the Americas. Following subcutaneous inoculation, Zika virus RNA was detected in plasma one day post-infection (dpi) in all animals (N = 8, including 2 animals infected during the first trimester of pregnancy). Plasma viral loads peaked above 1 x 105 viral RNA copies/mL in seven of eight animals. Viral RNA was also present in saliva, urine, and cerebrospinal fluid (CSF), consistent with case reports from infected humans. Viral RNA was cleared from plasma and urine by 21 dpi in non-pregnant animals. In contrast, both pregnant animals remained viremic longer, up to 57 days. In all animals, infection was associated with transient increases in proliferating natural killer cells, CD8+ T cells, CD4+ T cells, and plasmablasts. Neutralizing antibodies were detected in all animals by 21 dpi. Rechallenge of three non-pregnant animals with the Asian-lineage Zika virus 10 weeks after the initial challenge resulted in no detectable virus replication, suggesting that primary Zika virus infection elicits protective immunity against homologous virus strains. These data establish that Asian-lineage Zika virus infection of rhesus macaques provides a relevant animal model for studying pathogenesis in pregnant and non-pregnant individuals and evaluating potential interventions against human infection, including during pregnancy.
- Downloaded 1,530 times
- Download rankings, all-time:
- Site-wide: 9,093 out of 119,012
- In pathology: 46 out of 699
- Year to date:
- Site-wide: 69,942 out of 119,012
- Since beginning of last month:
- Site-wide: 78,762 out of 119,012
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!