Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 60,207 bioRxiv papers from 267,649 authors.

Entropic Forces Drive Clustering and Spatial Localization of Influenza A M2 During Viral Budding

By Jesper J. Madsen, John M A Grime, Jeremy S. Rossman, Gregory A Voth

Posted 28 Mar 2018
bioRxiv DOI: 10.1101/291120 (published DOI: 10.1073/pnas.1805443115)

The influenza A matrix 2 (M2) transmembrane protein facilitates virion release from the infected host cell. In particular, M2 plays a role in the induction of membrane curvature and/or in the scission process whereby the envelope is cut upon virion release. Here we show using coarse-grained computer simulations that various M2 assembly geometries emerge due to an entropic driving force, resulting in compact clusters or linearly extended aggregates as a direct consequence of the lateral membrane stresses. Conditions under which these protein assemblies will cause the lipid membrane to curve are explored and we predict that a critical cluster size is required for this to happen. We go on to demonstrate that under the stress conditions taking place in the cellular membrane as it undergoes large-scale membrane remodeling, the M2 protein will in principle be able to both contribute to curvature induction and sense curvature in order to line up in manifolds where local membrane line tension is high. M2 is found to exhibit linactant behavior in liquid-disordered/liquid-ordered phase-separated lipid mixtures and to be excluded from the liquid-ordered phase, in near-quantitative agreement with experimental observations. Our findings support a role for M2 in membrane remodeling during influenza viral budding both as an inducer and a sensor of membrane curvature, and they suggest a mechanism by which localization of M2 can occur as the virion assembles and releases from the host cell, independent of how the membrane curvature is produced.

Download data

  • Downloaded 406 times
  • Download rankings, all-time:
    • Site-wide: 23,419 out of 60,207
    • In biophysics: 869 out of 2,546
  • Year to date:
    • Site-wide: 39,926 out of 60,207
  • Since beginning of last month:
    • Site-wide: 43,771 out of 60,207

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News