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Reducing effects of particle adsorption to the air-water interface in cryoEM

By Alex J. Noble, Hui Wei, Venkata P Dandey, Zhening Zhang, Yong Zi Tan, Clinton S. Potter, Bridget Carragher

Posted 24 Mar 2018
bioRxiv DOI: 10.1101/288340

Most protein particles prepared in vitreous ice for single particle cryo-electron microscopy are adsorbed to air-water or substrate-water interfaces, potentially causing particles to adopt preferred orientations. Using the Spotiton robot and nanowire grids, we can reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrate this by using single particle cryoEM and cryoET on three biological samples.

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