Rxivist logo

Validating silicon polytrodes with paired juxtacellular recordings: method and dataset

By Joana Pereira Neto, Gonçalo Lopes, João Frazão, Joana Nogueira, Pedro Lacerda, Pedro Baião, Arno Aarts, Alexandru Andrei, Silke Musa, Elvira Fortunato, Pedro Barquinha, Adam Raymond Kampff

Posted 26 Jan 2016
bioRxiv DOI: 10.1101/037937 (published DOI: 10.1152/jn.00103.2016)

Cross-validating new methods for recording neural activity is necessary to accurately interpret and compare the signals they measure. Here we describe a procedure for precisely aligning two probes for in vivo paired-recordings such that the spiking activity of a single neuron is monitored with both a dense extracellular silicon polytrode and a juxtacellular micro-pipette. Our new method allows for efficient, reliable, and automated guidance of both probes to the same neural structure with micron resolution. We also describe a new dataset of paired-recordings, which is available online. We propose that our novel targeting system, and ever expanding cross-validation dataset, will be vital to the development of new algorithms for automatically detecting/sorting single-units, characterizing new electrode materials/designs, and resolving nagging questions regarding the origin and nature of extracellular neural signals.

Download data

  • Downloaded 1,575 times
  • Download rankings, all-time:
    • Site-wide: 10,765
    • In neuroscience: 1,174
  • Year to date:
    • Site-wide: 75,658
  • Since beginning of last month:
    • Site-wide: 74,397

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)